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KMID : 0385520090220060498
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Analytical Science & Technology
2009 Volume.22 No. 6 p.498 ~ p.503
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Chip-based isothermal amplification method for EGFR gene mutations in lung cancer
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Ahn Young-Chang
Park Su-Min
Seo Jae-Won
Yoon Il-Kyu
Jung Duck-Hyun
Lee Eun-Young
Nam Youn-Hyoung
Jang Won-Choeul
Kwon Pil-Seung
Kim Jong-Wan
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Abstract
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Lung cancer is the main cancer on the world today, due to the high case fatality. Lung cancer can devide into two major types, such as small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC). Mutations in the epidermal growth factor receptor (EGFR) have been described in patients with advanced NSCLC. Mutations in the EGFR are associated with clinical and radiographic responses to EGFR tyrosine kinase inhibitors gefitinib and erlotinib. Thus, the detection of EGFR mutation can offer an effective information in clinical decision-making. In this study, We developed very simple, cheep and rapid mutation detection system by chip-based isothermal amplification method. The method described here has shown the advantages of rapid amplification, high sensitivity, and specificity. Also, it will be useful for rapid and reliable clinical diagnosis of EGFR mutation.
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KEYWORD
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Chip, EGFR (Epidermal Growth Factor Receptor), Isothermal Amplification, Non-Small-Cell Lung Cancer (NSCLC)
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